Anti-alpha Adducin (phospho S59) 抗体 [EP2175AY] (ab76251)

製品の概要

  • 製品名Anti-alpha Adducin (phospho S59) antibody [EP2175AY]
    alpha Adducin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
  • 特異性ab76251 only detects alpha Adducin when phosphorylated at Serine 59.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin

  • ポジティブ・コントロール
    • HeLa cell lysates, untreated, or treated with calyculin A
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: 1/40.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
    Is unsuitable for Flow Cyt, ICC and IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
    • 組織特異性Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
    • 配列類似性Belongs to the aldolase class II family. Adducin subfamily.
    • ドメインEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
    • 細胞内局在Cytoplasm > cytoskeleton. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADD A antibody
      • ADD1 antibody
      • ADDA antibody
      • ADDA_HUMAN antibody
      • Adducin 1 (alpha) antibody
      • Alpha adducin antibody
      • Alpha-adducin antibody
      • Erythrocyte adducin alpha subunit antibody
      • Erythrocyte adducin subunit alpha antibody
      • MGC3339 antibody
      • MGC44427 antibody
      • OTTHUMP00000151163 antibody
      • OTTHUMP00000151164 antibody
      • OTTHUMP00000151165 antibody
      • OTTHUMP00000218664 antibody
      • OTTHUMP00000218665 antibody
      see all

    Anti-alpha Adducin (phospho S59) antibody [EP2175AY] 画像

    • All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) 使用論文

    ab76251 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76251.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"