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RabMAb

Anti-alpha Adducin (phospho S59) 抗体 [EP2175AY] (ab76251)

製品の概要

  • 製品名
    Anti-alpha Adducin (phospho S59) antibody [EP2175AY]
    alpha Adducin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
  • 特異性
    ab76251 only detects alpha Adducin when phosphorylated at Serine 59.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin

  • ポジティブ・コントロール
    • HeLa cell lysates, untreated, or treated with calyculin A
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: 1/40.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
    Is unsuitable for Flow Cyt, ICC and IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
    • 組織特異性
      Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
    • 配列類似性
      Belongs to the aldolase class II family. Adducin subfamily.
    • ドメイン
      Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
    • 細胞内局在
      Cytoplasm > cytoskeleton. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADD A antibody
      • ADD1 antibody
      • ADDA antibody
      • ADDA_HUMAN antibody
      • Adducin 1 (alpha) antibody
      • Alpha adducin antibody
      • Alpha-adducin antibody
      • Erythrocyte adducin alpha subunit antibody
      • Erythrocyte adducin subunit alpha antibody
      • MGC3339 antibody
      • MGC44427 antibody
      • OTTHUMP00000151163 antibody
      • OTTHUMP00000151164 antibody
      • OTTHUMP00000151165 antibody
      • OTTHUMP00000218664 antibody
      • OTTHUMP00000218665 antibody
      see all

    画像

    • All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab76251 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab76251.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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