Anti-alpha Adducin 抗体 (ab51130)
Key features and details
- Rabbit polyclonal to alpha Adducin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-alpha Adducin antibody
alpha Adducin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to alpha Adducin -
由来種
Rabbit -
特異性
There are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits. -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51130の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
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ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
組織特異性
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
配列類似性
Belongs to the aldolase class II family. Adducin subfamily. -
ドメイン
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
細胞内局在
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
- Unigene: 183706 Human
see all -
別名
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
画像
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All lanes : Anti-alpha Adducin antibody (ab51130)
Lane 1 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with with immunising peptide
Predicted band size: 81 kDa -
This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
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ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab51130 は 11 報の論文で使用されています。
- Moztarzadeh S et al. Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization. Sci Rep 12:14940 (2022). PubMed: 36056066
- Cabriel C et al. Combining 3D single molecule localization strategies for reproducible bioimaging. Nat Commun 10:1980 (2019). PubMed: 31040275
- Wang G et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife 8:N/A (2019). PubMed: 31042147
- Gervasi MG et al. The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure. J Cell Sci 131:N/A (2018). PubMed: 29739876
- Hauser M et al. The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage. Cell Rep 24:1512-1522 (2018). PubMed: 30089262