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Other Immunogen Type corresponding to alpha Adaptin. Purified adaptors.
Our Abpromise guarantee covers the use of ab2730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|Electron Microscopy||Use at an assay dependent concentration. PubMed: 23372794|
|ELISA||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/1000. This antibody detects an ~100 kDa doublet representing the two isoforms of alpha-adaptin in rat brain, though alpha C is the dominant form seen. Microinjection of cells with this antibody results in variable blockade of endocytosis.|
|IHC-FoFr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.|
Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Image from Li Y et al, J Biol Chem. 2012 Jan 2;287(1):523-30. Epub 2011 Nov 11, Fig 2. DOI 10.1074/jbc.M111.306183 January 2, 2012 The Journal of Biological Chemistry, 287, 523-530.ab2730 used in Western Blot at a 1/1000 dilution.