Anti-Alpha A + alpha B Crystallin 抗体 (ab28163)

製品の概要

  • 製品名
    Anti-Alpha A + alpha B Crystallin antibody
    Alpha A + alpha B Crystallin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Alpha A + alpha B Crystallin
  • 特異性
    ab28163 recognises alpha A and alpha B crystallin proteins. There is no reactivity with beta crystallin.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Cow, Human
  • 免疫原

    Synthetic peptides:

    EEKPTSAPSS

    conjugated to KLH, corresponding to amino acids 164/173 of Human alpha A; and REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163/175 of Human alpha B Crystallin

  • ポジティブ・コントロール
    • Bovine alpha Crystallin Protein, Bovine alpha A Crystallin Protein, Bovine alpha B Crystallin Protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 20 kDa.

This dilution was sufficient for detection of alpha A/beta B-crystallin in 20µg of alpha A-crystallin protein by colorimetric immunoblot analysis. Goat anti-Rabbit IgG-AP as secondary antibody.

ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Crystallins are separated into two classes: taxon specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Alpha crystallins are composed of two gene products: alpha A and alpha B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • Alpha crystallin A chain antibody
    • Alpha crystallin B chain antibody
    • CRYA1 antibody
    • CRYA2 antibody
    • CRYAA antibody
    • CRYAB antibody
    • Heat shock protein beta 4 antibody
    • Heat shock protein beta 5 antibody
    • HspB4 antibody
    • HspB5 antibody
    • Rosenthal fiber component antibody
    see all

Anti-Alpha A + alpha B Crystallin antibody 画像

  • All lanes : Anti-Alpha A + alpha B Crystallin antibody (ab28163) at 1/1000 dilution

    Lane 1 : Tissue lysates prepared from mouse heart
    Lane 2 : Cell lysates prepared from Hela cells
    Lane 3 : Crystallin alpha B native bovine protein
    Lane 4 : Crystallin alpha native bovine protein
    Lane 5 : Molecular weight marker


    Predicted band size : 20 kDa

プロトコール

Anti-Alpha A + alpha B Crystallin antibody (ab28163) 使用論文

ab28163 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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