Application
Western blot
Sample
Human Cell lysate - whole cell (Ovcar4 cells)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
15 µg
Specification
Ovcar4 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk / TBST
Dilution
1/10000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 50 kDa
Positive control
Lane 1- Ovcar4
Lane 2- Ovcar4
Lane 3- Ovcar4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Victoria Bridgeman
Verified customer
投稿 May 16 2016