FITC Anti-alpha Tubulin 抗体 [DM1A] - Microtubule Marker (ab64503)
Key features and details
- FITC Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
alpha Tubulin 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Gerbil -
免疫原
Full length native protein (purified) corresponding to alpha Tubulin.
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エピトープ
aa426-450. Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin. -
ポジティブ・コントロール
- ICC/IF: HeLa cells. IHC-Fr: Xenopus laevis stage 36 tissue. Flow Cyt (Intra): HeLa cells. Cultured human fibroblasts, baby hamster kidney (BHK) cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
DM1A -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
- Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
- Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
- HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (4) |
Use a concentration of 1 µg/ml.
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Flow Cyt (Intra) |
Use 5.1µl for 106 cells.
ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt (Intra)
Use 5.1µl for 106 cells. ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
see all -
別名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
画像
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ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab64503 は 17 報の論文で使用されています。
- Takagi T et al. Discovery of an F-actin-binding small molecule serving as a fluorescent probe and a scaffold for functional probes. Sci Adv 7:eabg8585 (2021). PubMed: 34797716
- Luo D et al. Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function. Front Cell Dev Biol 8:592433 (2020). PubMed: 33409275
- Eck F et al. ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis. J Cell Sci 133:N/A (2020). PubMed: 32843575
- Zheng N et al. Development of a Subcellular Semimechanism-Based Pharmacokinetic/Pharmacodynamic Model to Characterize Paclitaxel Effects Delivered by Polymeric Micelles. J Pharm Sci 108:725-731 (2019). PubMed: 30414867
- Zhou C et al. Substrate Compliance Directs the Osteogenic Lineages of Stem Cells from the Human Apical Papilla via the Processes of Mechanosensing and Mechanotransduction. ACS Appl Mater Interfaces 11:26448-26459 (2019). PubMed: 31251564