Application
Western blot
Sample
Chicken Tissue lysate - whole (Chicken Embryo)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Chicken Embryo
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST+5% milk
Dilution
1/10000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 57 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Yuval Cinnamon
Verified customer
投稿 Dec 12 2016