Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 (Human breast adenocarcinoma cell line))
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
20 µg
Specification
MCF7 (Human breast adenocarcinoma cell line)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0.5%, Milk 5%, NaN3
Dilution
1/200
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Clarity
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 19 kDa Non-specific: 40 kDa
Additional data
Additional Notes
Lane 1: Ladder
Lane 2: MCF7 (Human whole cell lysate)
No signal obtained in mouse, rat and human sample from intestinal tissue lysate (same western blot).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Julien Chevalier
Verified customer
投稿 Jul 20 2016