Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% TGX gel Biorad)
Sample
Human Cell lysate - whole cell (neuronal SHY5)
Specification
neuronal SHY5
Treatment
okadaic acid for 6-8 hours 37 oC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS Tw
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG1 HRP linked
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL GE Healthcare
Bands
Specific: 19 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Cells not treated with okadaic acid
Positive control
This was our positive control to make sure the antibody was working
since the brains we used from Parkinson transgenic mice did not
give any signal.
Additional data
Additional Notes
Have not published this yet but it is hard to find a good
commercial phospho S129 synuclein antibody so
we are very happy with this.
In fact we ordered another another AbCam S129 for human IHC
and it worked really well for presumed Lewy bodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Yien Kuo
Verified customer
投稿 Aug 30 2013