Application
Western blot
Sample
Sheep Tissue lysate - whole (Whole Placentome)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
100 µg
Specification
Whole Placentome
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Kirkegaard & Perry Labratories - Anti-Mouse IgG (H
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL on Kodak Biomax MR film
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 103 kDa Non-specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Bryan White
Verified customer
投稿 May 11 2011