Application
Western blot
Sample
Human Cell lysate - whole cell (Lung carcinoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
Lung carcinoma
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: Milk and PBS
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat Anti mouse Alkaline phosphatase conjugate
Host species: Goat
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
BCIP/NBT
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 105 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Apr 24 2009