Anti-alpha 5 Defensin 抗体 [8C8] (ab90802)

製品の概要

  • 製品名Anti-alpha 5 Defensin antibody [8C8]
    alpha 5 Defensin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8C8] to alpha 5 Defensin
  • 特異性ab90802 recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 has be detected.
  • アプリケーション適用あり: WB, IHC-Fr, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Recombinant fragment: ESLQERADEA TTQKQSGEDN QDLAISFAGN GLSALRTSGS QARATCYCRT GRCATRESLS GVCEISGRLY RLCCR, corresponding to amino acids 20-94 of Human alpha 5 Defensin purified from baculovirus infected Hi5 insect cells (UniProt Q01523).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)ab90802 was filtered through a 0.2 µm filter.
  • ポリ/モノモノクローナル
  • クローン名8C8
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50. Predicted molecular weight: 10 kDa.
IHC-Fr 1/50.
ELISA Use at an assay dependent concentration.
IHC-P 1/50.

ターゲット情報

  • 機能Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
  • 組織特異性Paneth cells of the small intestine (at protein level).
  • 配列類似性Belongs to the alpha-defensin family.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • Defa antibody
    • Defa29 antibody
    • DEFA5 antibody
    • Defcr29 antibody
    • Defcr5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Enteric defensin antibody
    • HD 5 antibody
    • HD5 antibody
    • HD5(20-94) antibody
    • HD5(63-94) antibody
    • MGC129728 antibody
    • RD 5 antibody
    see all

Anti-alpha 5 Defensin antibody [8C8] 画像

  • ab90802 at 1/50 dilution staining alpha 5 Defensin in human Ileus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). 

Anti-alpha 5 Defensin antibody [8C8] (ab90802) 使用論文

This product has been referenced in:
  • Spencer JD  et al. Human alpha defensin 5 expression in the human kidney and urinary tract. PLoS One 7:e31712 (2012). IHC ; Human . Read more (PubMed: 22359618) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Ileum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification Ileum
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Dr. B Pahar

Verified customer

投稿 Jan 29 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ileum human)
Specification Ileum human
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval buffer pH 6.0
Permeabilization No
Blocking step 70% Ethanol with H2O2 as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Dr. Ilka Werner-Martini

Verified customer

投稿 Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"