Anti-alpha 5 Defensin 抗体 [8C8] (ab90802)

製品の概要

  • 製品名
    Anti-alpha 5 Defensin antibody [8C8]
    alpha 5 Defensin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8C8] to alpha 5 Defensin
  • 特異性
    ab90802 recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 has be detected.
  • アプリケーション
    適用あり: WB, IHC-Fr, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Recombinant fragment: ESLQERADEA TTQKQSGEDN QDLAISFAGN GLSALRTSGS QARATCYCRT GRCATRESLS GVCEISGRLY RLCCR, corresponding to amino acids 20-94 of Human alpha 5 Defensin purified from baculovirus infected Hi5 insect cells (UniProt Q01523).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab90802 was filtered through a 0.2 µm filter.
  • ポリ/モノ
    モノクローナル
  • クローン名
    8C8
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50. Predicted molecular weight: 10 kDa.
IHC-Fr 1/50.
ELISA Use at an assay dependent concentration.
IHC-P 1/50.

ターゲット情報

  • 機能
    Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
  • 組織特異性
    Paneth cells of the small intestine (at protein level).
  • 配列類似性
    Belongs to the alpha-defensin family.
  • 翻訳後修飾
    Glycosylated.
  • 細胞内局在
    Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • Defa antibody
    • Defa29 antibody
    • DEFA5 antibody
    • Defcr29 antibody
    • Defcr5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Enteric defensin antibody
    • HD 5 antibody
    • HD5 antibody
    • HD5(20-94) antibody
    • HD5(63-94) antibody
    • MGC129728 antibody
    • RD 5 antibody
    see all

画像

  • ab90802 at 1/50 dilution staining alpha 5 Defensin in human Ileus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). 

参考文献

This product has been referenced in:
  • Spencer JD  et al. Human alpha defensin 5 expression in the human kidney and urinary tract. PLoS One 7:e31712 (2012). IHC ; Human . Read more (PubMed: 22359618) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification
Ileum
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Bapi Pahar

Verified customer

投稿 Jan 29 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ileum human)
Specification
Ileum human
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval buffer pH 6.0
Permeabilization
No
Blocking step
70% Ethanol with H2O2 as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Dr. Ilka Werner-Martini

Verified customer

投稿 Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録