Anti-alpha 2 Macroglobulin 抗体 (ab84176)

製品の概要

  • 製品名Anti-alpha 2 Macroglobulin antibody
    alpha 2 Macroglobulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to alpha 2 Macroglobulin
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1450 to the C-terminus of Human alpha 2 Macroglobulin.

    (Peptide available as ab93750.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both human plasma total protein and human placenta tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 185 kDa (predicted molecular weight: 163 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 組織特異性Secreted in plasma.
  • 配列類似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 発生段階Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A2m antibody
    • A2MG_HUMAN antibody
    • Alpha 2 M antibody
    • Alpha 2M antibody
    • Alpha-2-M antibody
    • Alpha-2-macroglobulin antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
    • CPAMD5 antibody
    • DKFZp779B086 antibody
    • FWP007 antibody
    • S863 7 antibody
    see all

Anti-alpha 2 Macroglobulin antibody 画像

  • All lanes : Anti-alpha 2 Macroglobulin antibody (ab84176) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 163 kDa
    Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab84176 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

Anti-alpha 2 Macroglobulin antibody (ab84176) 使用論文

ab84176 has not yet been referenced specifically in any publications.

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Read More
Application Western blot
Sample Human Tissue lysate - whole (human heart left ventricular)
Loading amount 40 µg
Specification human heart left ventricular
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Dawn Bowles

Verified customer

投稿 Jan 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"