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Our Abpromise guarantee covers the use of ab58703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab58703 staining alpha 2 Macroglobulin in rat decidual cells by Immunocytochemistry. Cells were fixed with Methanol, permeabilized with PBS, 5% BSA, 0.1% Triton X-100 and 0.2% Tween-20 and blocking with 5% BSA was performed for 15 minutes at RT. Samples were incubated with primary antibody (1/500) in PBS for 14 hours at 4°C. A commercially available Avidin–biotin alkaline phosphatase complex technique was used.
Image from Jain MR et al, Proteome Sci. 2009 Jul 16;7:25, Fig 2.