Anti-alpha 2 Glycine Receptor 抗体 (ab97628)
製品の概要
-
製品名Anti-alpha 2 Glycine Receptor antibody
-
製品の詳細Rabbit polyclonal to alpha 2 Glycine Receptor
-
由来種Rabbit
-
アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
-
種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
-
免疫原
Recombinant protein fragment corresponding to a region within N terminal amino acids 1 and 186 of alpha 2 Glycine Receptor.
-
ポジティブ･コントロール
- ICC/IF: formaldehyde fixed SKNSH cells. WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 or Raji cell lysates. IHC-P: MDAMB468 xenograft.
法規制情報
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab97628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 52 kDa. | |
| IHC-P | 1/100 - 1/500. |
ターゲット情報
-
機能The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
-
配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA2 sub-subfamily.
-
細胞内局在Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
-
参照データベース
- Entrez Gene: 2742 Human
- Entrez Gene: 237213 Mouse
- Entrez Gene: 24397 Rat
- Omim: 305990 Human
- SwissProt: P23416 Human
- SwissProt: Q7TNC8 Mouse
- SwissProt: P22771 Rat
- Unigene: 2700 Human
see all -
別名
- GLR antibody
- Glra2 antibody
- GLRA2_HUMAN antibody
see all
画像
-
Western blot - Anti-alpha 2 Glycine Receptor antibody (ab97628)Anti-alpha 2 Glycine Receptor antibody (ab97628) at 1/5000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10 % SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Glycine Receptor antibody (ab97628)ab97628, at a 1/500 dilution, staining alpha 2 Glycine Receptor in paraffin embedded MDAMB468 xenograft by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-alpha 2 Glycine Receptor antibody (ab97628)ICC/IF image of ab97628 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97628 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Ren M et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . Read more (PubMed: 27112667) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"