Customer reviews and FAQs

製品の概要

  • 製品名
    Anti-alpha 2 Glycine Receptor antibody
  • 製品の詳細
    Rabbit polyclonal to alpha 2 Glycine Receptor
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment corresponding to a region within N terminal amino acids 1 and 186 of alpha 2 Glycine Receptor.

  • ポジティブ･コントロール
    • ICC/IF: formaldehyde fixed SKNSH cells. WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 or Raji cell lysates. IHC-P: MDAMB468 xenograft.

法規制情報

医薬用外毒物

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
  • 配列類似性
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA2 sub-subfamily.
  • 細胞内局在
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • GLR antibody
    • Glra2 antibody
    • GLRA2_HUMAN antibody
    • Glycine receptor alpha 2 polypeptide antibody
    • Glycine receptor alpha 2 subunit antibody
    • Glycine receptor subunit alpha 2 antibody
    • Glycine receptor subunit alpha-2 antibody
    • Gycine receptor alpha 2 antibody
    see all

Anti-alpha 2 Glycine Receptor antibody 画像

  • Western blot - Anti-alpha 2 Glycine Receptor antibody (ab97628)
    Western blot - Anti-alpha 2 Glycine Receptor antibody (ab97628)
    Anti-alpha 2 Glycine Receptor antibody (ab97628) at 1/5000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
    10 % SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Glycine Receptor antibody (ab97628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Glycine Receptor antibody (ab97628)
    ab97628, at a 1/500 dilution, staining alpha 2 Glycine Receptor in paraffin embedded MDAMB468 xenograft by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 2 Glycine Receptor antibody (ab97628)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 2 Glycine Receptor antibody (ab97628)
    ICC/IF image of ab97628 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97628 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha 2 Glycine Receptor antibody (ab97628) 使用論文

This product has been referenced in:
  • Ren M  et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . Read more (PubMed: 27112667) »

See 1 Publication for this product

ab97628 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab97628 の abreview を投稿する ab97628 に関する問い合わせをする

1 Abreview or Q&A

10
Votes: Highest First

I'm seeing multiple bands in WB using this antibody and mouse skin tissue.  I'm seeing bands around 40 & 60 kDa, but nothing around 50 kDa. I would also like to test ab97628 in mouse, an untested species.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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