Anti-alpha-N-Catenin recognizes alpha-N-catenin (102 kDa) by immunoblotting.
Staining of the alpha-N-catenin band in immunoblotting is specifically inhibited with the alpha-N-catenin immunizing peptide (human, amino acids 171-186).
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000. Detects a band of approximately 102 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system. Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation.
Belongs to the vinculin/alpha-catenin family.
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell projection > axon. Recruited to the nucleus upon ZNF639 overexpression.
Immunohistochemistry (Frozen sections) - Anti-alpha 2 Catenin antibody (ab11347)Image from Stocker AM et al., Brain Res. 2006;16:151-8. Fig 4.; doi: 10.1016/j.brainres.2005.12.057 with permission from Elsevier.
ab11347 staining alpha 2 Catenin (left) in Mouse cortical ventricular zone tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde in PBS overnight at 4°C, then saturated in 30% sucrose at 4°C (for at least 18 hours). Fixed and saturated brains were embedded in OCT compound and frozen rapidly with dry ice, and then 12µm cyrosections collected and stored at -80°C. Samples were incubated with primary antibody (1/500). Middle - non-phosphorylated active beta catenin, Right - merge. Beta catenin co-localizes with alpha catenin at the apical junctions rings.
Butko MT et al. In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation. Proc Natl Acad Sci U S A110:E726-35 (2013).
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Thompson J et al. Adherens junction breakdown in the periderm following cadmium administration in the chick embryo: distribution of cadherins and associated molecules. Reprod Toxicol25:39-46 (2008).
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