Anti-alpha 1b Adrenergic Receptor 抗体 (ab84405)

製品の概要

  • 製品名
    Anti-alpha 1b Adrenergic Receptor antibody
    alpha 1b Adrenergic Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to alpha 1b Adrenergic Receptor
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A 15 amino acid peptide from the C terminal residues of human alpha 1b Adrenergic Receptor (NM_000679; P35368).

  • ポジティブ・コントロール
    • Human fetal brain lysates; human heart tissue.

法規制情報

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab84405 is purified by a peptide affinity column.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human alpha 1b Adrenergic Receptor peptide (ab226906).
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
  • 細胞内局在
    Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADA1B_HUMAN antibody
    • ADRA 1 antibody
    • Adra 1b antibody
    • ADRA1 antibody
    • ADRA1B antibody
    • Adrenergic alpha 1B receptor antibody
    • Adrenergic receptor alpha 1b antibody
    • Adrenoceptor alpha 1B antibody
    • Alpha 1 adrenergic receptor antibody
    • Alpha 1B adrenoceptor antibody
    • Alpha 1B adrenoreceptor antibody
    • Alpha-1B adrenergic receptor antibody
    • Alpha-1B adrenoceptor antibody
    • Alpha-1B adrenoreceptor antibody
    • Alpha1 adrenergic receptor antibody
    • Alpha1B adrenergic receptor antibody
    • Alpha1BAR antibody
    see all

画像

  • Anti-alpha 1b Adrenergic Receptor antibody (ab84405) at 1/500 dilution + human fetal brain lysates

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ab84405, at a 1/100 dilution, staining alpha 1b Adrenergic Receptor in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.
  • ICC/IF image of ab84450 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84450, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Mitrano DA  et al. D1-dopamine and a1-adrenergic receptors co-localize in dendrites of the rat prefrontal cortex. Neuroscience 258:90-100 (2014). ICC/IF ; Human . Read more (PubMed: 24231738) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Username

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投稿 Aug 18 2014

Thank you for your response. Regrettably, ab12923, ab12935 and ab13298 have been discontinued so currently we do no have stock of them in our catalogue. I have conducted a search for your and found some alternatives products for these targets. ...

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