Anti-alpha 1 Spectrin 抗体 [17C7] (ab11751)

製品の概要

  • 製品名
    Anti-alpha 1 Spectrin antibody [17C7]
    alpha 1 Spectrin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [17C7] to alpha 1 Spectrin
  • 特異性
    ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.
  • アプリケーション
    適用あり: ICC/IF, ELISA, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

  • ポジティブ・コントロール
    • Erythroid spectrin in red blood cells and cerebellum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100. (See Abreview)
ELISA 1/100000 - 1/1e+006.
WB 1/100 - 1/2000. Predicted molecular weight: 280 kDa. PubMed: 23780662
IHC-Fr 1/10 - 1/50.

ターゲット情報

  • 機能
    Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
  • 関連疾患
    Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
    Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
    Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.
  • 配列類似性
    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 21 spectrin repeats.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha I spectrin antibody
    • EL 2 antibody
    • EL2 antibody
    • Elliptocytosis 2 antibody
    • Elliptocytosis2 antibody
    • Erythrocyte alpha spectrin antibody
    • erythrocyte antibody
    • Erythroid alpha spectrin antibody
    • Erythroid alpha-spectrin antibody
    • Erythroid spectrin alpha antibody
    • HPP antibody
    • HS3 antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain erythrocyte antibody
    • Spectrin alpha erythrocytic 1 antibody
    • SPH3 antibody
    • SPTA 1 antibody
    • SPTA antibody
    • SPTA1 antibody
    • SPTA1_HUMAN antibody
    see all

画像

  • ab11751 staining alpha 1 Spectrin in rat artery smooth muscle cells by Immunocytochemistry/ Immunofluorescence
    Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and then incubated with ab11751 used at a 1/100 dilution for 1 hour at 21°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse monoclonal used at a 1/100 dilution.

    Rat artery smooth muscle cells were triple stained for spectrin (green), Actin (red) and DNA blue. The cell in the image is in telophase and spectrin is localized at the midbody.

    See Abreview

  • Central Panel: ab11751 staining alpha 1 Spectrin in human PMN cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/250, was used as secondary antibody.
    Left-hand panel: Nuclei counterstained with DAPI (blue).
    Right-hand panel: Overlay

    See Abreview

  • Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg

    Secondary
    HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 280 kDa
    Additional bands at : 200 kDa (possible cleavage fragment).

    Exposure time : 1 hour

    This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa

    See Abreview

参考文献

This product has been referenced in:
  • Watermeyer JM  et al. A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes. Blood 127:343-51 (2016). Read more (PubMed: 26637786) »
  • Gawinecka J  et al. Subtype-specific synaptic proteome alterations in sporadic Creutzfeldt-Jakob disease. J Alzheimers Dis 37:51-61 (2013). WB ; Human . Read more (PubMed: 23780662) »

See all 7 Publications for this product

レビューと Q&A

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab11751 has never been tested in immunohisochemistry on paraffin embedded sections (IHC-P). All tested and guaranteed applications and speciesare specified on our...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat artery smooth muscle cells)
Specification
rat artery smooth muscle cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton x100
Username

Dr. Rosalind Silverman-Gavrila

Verified customer

投稿 Jan 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelet Lysate (precleared))
Loading amount
25 µg
Specification
Platelet Lysate (precleared)
Gel Running Conditions
Reduced Denaturing (4-15)
Blocking step
Thermo Starting Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Jan 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PMN)
Specification
PMN
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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