Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) 抗体 [EPR2375(2)] (ab124677)

製品の概要

  • 製品名Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)]
    alpha 1 Sodium Potassium ATPase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2375(2)] to alpha 1 Sodium Potassium ATPase (phospho Y10)
  • 特異性ab124677 only detects alpha 1 Sodium Potassium ATPase phosphorylated at Tyrosine 10.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Tyrosine 10 of Human alpha 1 Sodium Potassium ATPase.

  • ポジティブ・コントロール
    • HeLa cell lysate, treated with Pervanadate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR2375(2)
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 113 kDa).
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
    • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
    • 翻訳後修飾Phosphorylation on Tyr-10 modulates pumping activity.
    • 細胞内局在Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A1A1 antibody
      • AT1A1 antibody
      • AT1A1_HUMAN antibody
      • ATP1A1 antibody
      • Atpa-1 antibody
      • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
      • ATPase Na+/K+ transporting subunit alpha 1 antibody
      • BC010319 antibody
      • EC 3.6.3.9 antibody
      • MGC3285 antibody
      • MGC38419 antibody
      • MGC51750 antibody
      • Na K ATPase alpha A catalytic polypeptide antibody
      • Na K ATPase catalytic subunit alpha A protein antibody
      • Na(+)/K(+) ATPase 1 antibody
      • Na(+)/K(+) ATPase alpha-1 subunit antibody
      • Na+, K+ ATPase alpha subunit antibody
      • Na+/K+ ATPase alpha 1 subunit antibody
      • Na+/K+ ATPase 1 antibody
      • Na,K ATPase alpha 1 subunit antibody
      • Nkaa1b antibody
      • Sodium potassium ATPase alpha 1 polypeptide antibody
      • Sodium pump 1 antibody
      • Sodium pump subunit alpha-1 antibody
      • sodium-potassium ATPase catalytic subunit alpha-1 antibody
      • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
      see all

    Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] 画像

    • All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate, treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 113 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] (ab124677) 使用論文

    ab124677 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"