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Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Rabbit renal outermedulla.
Our Abpromise guarantee covers the use of ab7671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.
We recommend Methanol fixation
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.|
|Flow Cyt||Use a concentration of 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody 10 µg/ml in PBS for 1 hour. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.
ab7671 staining (dog) MDCK II cells by ICC/IF. Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.
ab7671 staining Sodium Potassium ATPase in 293 human embryonic kidneys cells. Cells were plated on an uncoated cover slip. One day after plating cells were washed twice with PBS and fixed with 4% paraformaldehyde with 4% sucrose at room temperature for 15 minutes. After washing once with PBS, 300µl wheat germ agglutinin (5µg/ml in PBS) conjugated to TexasRed were added and incubated at 4°C in darkness for 15 minutes. Cells were then washed twice with PBS and permeabilized with 0.5% saponin in PBS for 45 minutes at 4°C. Blocking was carried out with 10% fetal calf serum in PBS-saponin during first and second antibody incubation. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/500 dilution. After additional washing cover slips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI.
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