Anti-alpha 1 Sodium Potassium ATPase 抗体 [464.6] (ab7671)

製品の概要

  • 製品名Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
    alpha 1 Sodium Potassium ATPase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
  • 特異性This antibody is specific for Na,K-ATPase alpha 1 subunit.
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Dog, Human, Pig, Xenopus laevis, Monkey
  • 免疫原

    Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Rabbit renal outermedulla.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 - 10 µg/ml.

We recommend Methanol fixation

WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
Flow Cyt Use a concentration of 10 µg/ml.

See Abreview.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • 翻訳後修飾Phosphorylation on Tyr-10 modulates pumping activity.
  • 細胞内局在Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] 画像

  • Lanes 1 - 3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
    Lanes 4 - 6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
    Lanes 7 - 9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 6 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 7 : Human brain tissue lysate - total protein (ab29466)
    Lane 8 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 9 : Brain (Rat) Tissue Lysate (ab7942)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa


    Exposure time : 3 minutes
  • ab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab7671 staining Sodium Potassium ATPase - Plasma Membrane Marker in Human Fibrosarcoma HT1080 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The  sample was incubated with the primary antibody 10 µg/ml in PBS  for 1 hour. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.

    See Abreview

  • All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ab7671 staining (dog) MDCK II cells by ICC/IF.  Cells were acetone fixed and blocked with 4% serum for 10 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C.  Ab6785 (a FITC conjugated goat polyclonal to mouse IgG - H&L) was diluted 1/100 and employed as the secondary antibody.

    See Abreview

  • Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate

    Predicted band size : 112 kDa
    Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.
  • ab7671 staining Sodium Potassium ATPase in 293 human embryonic kidneys cells. Cells were plated on an uncoated cover slip. One day after plating cells were washed twice with PBS and fixed with 4% paraformaldehyde with 4% sucrose at room temperature for 15 minutes. After washing once with PBS, 300µl wheat germ agglutinin (5µg/ml in PBS) conjugated to TexasRed were added and incubated at 4°C in darkness for 15 minutes. Cells were then washed twice with PBS and permeabilized with 0.5% saponin in PBS for 45 minutes at 4°C. Blocking was carried out with 10% fetal calf serum in PBS-saponin during first and second antibody incubation. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/500 dilution. After additional washing cover slips were mounted on glass slides with ProLong® Gold antifade reagent with DAPI.

    See Abreview

  • ICC/IF image of ab7671 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7671, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.
  • All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/1000 dilution

    Lane 1 : Parotid acinar cell lysate
    Lane 2 : Parotid acinar cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-Mouse polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 112 kDa


    Exposure time : 5 minutes

    See Abreview

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) 使用論文

This product has been referenced in:
  • Chen C  et al. Epigenetic modification of PKM? rescues aging-related cognitive impairment. Sci Rep 6:22096 (2016). WB . Read more (PubMed: 26926225) »
  • Shimizu H  et al. Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel ß subunits. Sci Rep 6:26618 (2016). Read more (PubMed: 27216889) »

See all 67 Publications for this product

Product Wall

Thank you for your response.

Our Lab regularly test this product on whole cell lysates and heat the samples 95-100C for 10 minutes. The signal is very clear and distinctive. My personal experience is that plasmamebrane protein can be heating ...

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Application Western blot
Sample Human Cell lysate - whole cell (OV90 cells)
Gel Running Conditions Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount 1e+006 cells
Specification OV90 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Dec 06 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (N87 cell line)
Permeabilization Yes - 0.25% Triton X-100
Specification N87 cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative Formaldehyde
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投稿 Sep 14 2016

Application Western blot
Sample Rat Cell lysate - whole cell (cortex)
Gel Running Conditions Reduced Denaturing (7%)
Loading amount 10 µg
Specification cortex
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
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投稿 Mar 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - other (canine dermal fibroblasts-membrane fraction)
Gel Running Conditions Reduced Denaturing (7.5%)
Loading amount 10 µg
Specification canine dermal fibroblasts-membrane fraction
Blocking step Block ace as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Mar 11 2016

Application Western blot
Sample Rat Cell lysate - whole cell (Lung)
Gel Running Conditions Reduced Denaturing
Loading amount 10000 cells
Specification Lung
Blocking step (agent) for 30 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Oct 08 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Lung)
Specification Lung
Blocking step BSA as blocking agent for 1 minute(s) · Concentration: 500% · Temperature: 22°C
Fixative Methanol
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投稿 Jul 10 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (14 days old embryonic brain section, 16 micron)
Specification 14 days old embryonic brain section, 16 micron
Permeabilization Yes - Triton X100
Fixative Paraformaldehyde
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投稿 Jan 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ph 9 steamer
Sample Human Tissue sections (small bowel)
Specification small bowel
Permeabilization No
Fixative Formaldehyde
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投稿 Dec 05 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Hepatocytes)
Specification Hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jun 30 2014

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