Anti-alpha 1 Fetoprotein 抗体 (ab54745)

製品の概要

  • 製品名Anti-alpha 1 Fetoprotein antibody
    alpha 1 Fetoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to alpha 1 Fetoprotein
  • アプリケーション適用あり: WB, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 500-610 of Human alpha 1 Fetoprotein

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab54745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P Use a concentration of 3 µg/ml.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to alpha 1 Fetoprotein (ab8201). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 組織特異性Plasma. Synthesized by the fetal liver and yolk sac.
  • 配列類似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 発生段階Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻訳後修飾Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody 画像

  • alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 69 kDa
    alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ab54745 at 10 ug/ml staining alpha Fetoprotein in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml

プロトコール

Anti-alpha 1 Fetoprotein antibody (ab54745) 使用論文

ab54745 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Leica's universal IHC blockig/diluent solution as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Sample Cat Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Formaldehyde
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投稿 Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"