Anti-alpha 1 Fetoprotein 抗体 [143CT50.11.8.1] (ab86583)

製品の概要

  • 製品名
    Anti-alpha 1 Fetoprotein antibody [143CT50.11.8.1]
    alpha 1 Fetoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [143CT50.11.8.1] to alpha 1 Fetoprotein
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human alpha 1 Fetoprotein.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Hepg2 whole cell, MCF7 whole cell, Human Ovary tissue.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

ターゲット情報

  • 機能
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 組織特異性
    Plasma. Synthesized by the fetal liver and yolk sac.
  • 配列類似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 発生段階
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻訳後修飾
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

画像

  • All lanes : Anti-alpha 1 Fetoprotein antibody [143CT50.11.8.1] (ab86583) at 5 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 69 kDa


    Exposure time : 20 minutes

プロトコール

参考文献

ab86583 has not yet been referenced specifically in any publications.

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