Anti-alpha 1 Catenin 抗体 [EP1793Y] (ab51032)

製品の概要

  • 製品名Anti-alpha 1 Catenin antibody [EP1793Y]
    alpha 1 Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1793Y] to alpha 1 Catenin
  • アプリケーション適用あり: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of human alpha 1 Catenin.

  • ポジティブ・コントロール
    • HeLa cell lysate, human breast carcinoma tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IP 1/50.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • 組織特異性Expressed ubiquitously in normal tissues.
  • 配列類似性Belongs to the vinculin/alpha-catenin family.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Anti-alpha 1 Catenin antibody [EP1793Y] 画像

  • Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • Ab51032 (1:100) staining human alpha 1 Catenin in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
  • ICC/IF image of ab51032 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51032, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (IgG H+L; ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ICC/IF image of Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) stained mouse ES cells. The cells were fixed in 1:1 methanol/acetone, permeabilized using 0.1% Triton X, and blocked with 1% serum in PBS for 30 minutes. The cells were then incubated with ab51032 at a 1/100 dilution for 16 hours at 4oC. The secondary antibody was a Goat Anti-Rabbit Alexa Fluor 488 (IgG H&L;  ab150077) used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing MCF7 cells stained with ab51032 (red line). The cells were fixed with 4% PFA (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51032, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-rabbit DyLight® 488 (IgG H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa


    Exposure time : 1 minute

Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) 使用論文

This product has been referenced in:
  • Dietrich JE  et al. Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages. EMBO Rep 16:1005-21 (2015). Read more (PubMed: 26142281) »
  • Giampietro C  et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol 211:1177-92 (2015). Read more (PubMed: 26668327) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Undifferentiated Human ES cells on MEFs)
Permeabilization Yes - see blocking buffer
Specification Undifferentiated Human ES cells on MEFs
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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投稿 Feb 01 2016

Application Western blot
Sample Human Cell lysate - whole cell (Undifferentiated Human ES cells on MEFs)
Gel Running Conditions Non-reduced Denaturing (12.5% SDS gel)
Loading amount 20 µg
Specification Undifferentiated Human ES cells on MEFs
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Feb 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Nov 13 2015

Application Western blot
Sample Human Cell lysate - whole cell (hESC, MCF7, MET5A)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 100000 cells
Specification hESC, MCF7, MET5A
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Aug 18 2015

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0.5% v/v tween20 in PBS
Sample Mouse Cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Gating Strategy live cells in fsc vs ssc
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.5% v/v tween20 in PBS
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投稿 Apr 30 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Dec 30 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample Mouse Cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Permeabilization Yes - 0.1% triton x
Fixative methanol/acetone 1:1
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投稿 Dec 20 2013

Thank you for contacting us.

This product was tested with rat, human and mouse samples only; xenopus was never tried. We can however predict, it will be fine for Xenopus as well because the N-terminal end of Xenopus protein (http://www.uniprot...

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Thank you for contacting Abcam. I would recommend ab7671, the alpha 1 Sodium Potassium ATPase antibody as a marker for your membrane fraction. Please let me know if there is anything else I can help you with.

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