製品の概要
- 製品名Anti-alpha 1 Catenin antibody [EP1793Y]
alpha 1 Catenin 一次抗体 製品一覧 - 製品の詳細Rabbit monoclonal [EP1793Y] to alpha 1 Catenin
- アプリケーション適用あり: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
- 種交差性交差種: Mouse, Rat, Human
- 免疫原
Synthetic peptide corresponding to residues near the N-terminus of human alpha 1 Catenin.
- ポジティブ･コントロール
- HeLa cell lysate, human breast carcinoma tissue.
- 特記事項
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- 精製度Tissue culture supernatant
- ポリ/モノモノクローナル
- クローン名EP1793Y
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab51032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). | |
| IP | 1/50. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
- 機能Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
- 組織特異性Expressed ubiquitously in normal tissues.
- 配列類似性Belongs to the vinculin/alpha-catenin family.
- 翻訳後修飾Sumoylated.
- 細胞内局在Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
- Information by UniProt
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参照データベース
- Entrez Gene: 1495 Human
- Entrez Gene: 12385 Mouse
- Entrez Gene: 307505 Rat
- Omim: 116805 Human
- SwissProt: P35221 Human
- SwissProt: P26231 Mouse
- Unigene: 445981 Human
- Unigene: 656653 Human
see all -
別名
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
see all
Anti-alpha 1 Catenin antibody [EP1793Y] 画像
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![Western blot - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)](http://a.abcam.com/ps/products/51/ab51032/Images/ab51032_1.bmp)
Western blot - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size : 100 kDa
Observed band size : 100 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)](http://a.abcam.com/ps/products/51/ab51032/Images/ab51032_2.bmp)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)Ab51032 (1:100) staining human alpha 1 Catenin in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue. -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)](http://a.abcam.com/ps/products/51/ab51032/Images/alpha-1-Catenin-Primary-antibodies-ab51032-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)ICC/IF image of ab51032 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51032, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (IgG H+L; ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)](http://a.abcam.com/ps/products/51/ab51032/Images/ab51032-215308-ab5103240228copy.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)This image is courtesy of an anonymous abreview.ICC/IF image of Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) stained mouse ES cells. The cells were fixed in 1:1 methanol/acetone, permeabilized using 0.1% Triton X, and blocked with 1% serum in PBS for 30 minutes. The cells were then incubated with ab51032 at a 1/100 dilution for 16 hours at 4oC. The secondary antibody was a Goat Anti-Rabbit Alexa Fluor 488 (IgG H&L; ab150077) used at a 1/500 dilution.
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![Flow Cytometry - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)](http://a.abcam.com/ps/products/51/ab51032/Images/alpha-1-Catenin-Primary-antibodies-ab51032-2.jpg)
Flow Cytometry - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)Overlay histogram showing MCF7 cells stained with ab51032 (red line). The cells were fixed with 4% PFA (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51032, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-rabbit DyLight® 488 (IgG H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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Western blot
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 100 kDa -
Western blot - Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032)
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 100 kDa
Exposure time : 1 minute
Anti-alpha 1 Catenin antibody [EP1793Y] (ab51032) 使用論文
This product has been referenced in:
- Qi L et al. TRIM16 suppresses the progression of prostate tumors by inhibiting the Snail signaling pathway. Int J Mol Med 38:1734-1742 (2016). WB ; Human . Read more (PubMed: 27748839) »
- Dietrich JE et al. Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages. EMBO Rep 16:1005-21 (2015). Read more (PubMed: 26142281) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"