製品の概要

  • 製品名Anti-alpha 1 Catenin antibody [25B1]
    alpha 1 Catenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [25B1] to alpha 1 Catenin
  • 特異性Human alpha 1 Catenin
  • アプリケーション適用あり: IHC-Fr, Flow Cyt, ICC/IF, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Prokaryotic recombinant protein corresponding to a 400 amino acid portion of the N terminal region of the Human alpha 1 Catenin molecule.

  • ポジティブ・コントロール
    • IHC: Human tonsil or bowel tissue. WB: HeLa cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa. IHC-P (FFPE): Human Colon (Normal) tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50. Acetone fixation recommended.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
IP Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 100 kDa.
IHC-P Use at an assay dependent concentration. High temperature antigen unmasking technique using 1 mM EDTA (pH 8.0) recommended.

ターゲット情報

  • 機能Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
  • 組織特異性Expressed ubiquitously in normal tissues.
  • 配列類似性Belongs to the vinculin/alpha-catenin family.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha E-catenin antibody
    • Cadherin associated protein 102kDa antibody
    • Cadherin associated protein antibody
    • Cadherin-associated protein antibody
    • CAP 102 antibody
    • CAP102 antibody
    • Catenin (cadherin associated protein) alpha 1 102kDa antibody
    • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
    • Catenin alpha 1 antibody
    • Catenin alpha-1 antibody
    • CTNA1_HUMAN antibody
    • CTNNA 1 antibody
    • Ctnna1 antibody
    • FLJ36832 antibody
    • FLJ52416 antibody
    • NY REN 13 antigen antibody
    • OTTHUMP00000224141 antibody
    • OTTHUMP00000224147 antibody
    • Renal carcinoma antigen NY REN 13 antibody
    • Renal carcinoma antigen NY-REN-13 antibody
    see all

Anti-alpha 1 Catenin antibody [25B1] 画像

  • alpha 1 Catenin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49105.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 100kDa; alpha 1 Catenin

  • IHC image of alpha 1 Catenin staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49105, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ICC/IF image of ab49105 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab49105 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical staining of alpha catenin in paraffin embedded human appendix tissue using ab49105 at 1:50 dilution following high temperature antigen unmasking technique using 1mM EDTA (pH8.0). 60 minutes primary antibody incubation at 25oC. Standard ABC technique. Note intense membrane staining of the mucosal epithelial cells.
  • Overlay histogram showing HeLa cells stained with ab49105 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41905, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-alpha 1 Catenin antibody [25B1] (ab49105) 使用論文

This product has been referenced in:
  • Fiore C  et al. Utility of multispectral imaging in automated quantitative scoring of immunohistochemistry. J Clin Pathol 65:496-502 (2012). IHC-P ; Human . Read more (PubMed: 22447914) »
  • Rakha EA  et al. Clinical and biological significance of E-cadherin protein expression in invasive lobular carcinoma of the breast. Am J Surg Pathol 34:1472-9 (2010). Read more (PubMed: 20871222) »

See all 2 Publications for this product

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