製品の概要

  • 製品名Anti-alpha 1 Antitrypsin antibody [G11]
    alpha 1 Antitrypsin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [G11] to alpha 1 Antitrypsin
  • 特異性Recognises alpha 1-antitrypsin. Does not react with other human plasma proteins.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.

ターゲット情報

  • 機能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 組織特異性Ubiquitous. Expressed in leukocytes and plasma.
  • 関連疾患Alpha-1-antitrypsin deficiency
  • 配列類似性Belongs to the serpin family.
  • ドメインThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻訳後修飾N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 細胞内局在Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Dom1 antibody
    • PI antibody
    • PI1 antibody
    • PRO2275 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • Serpina1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Anti-alpha 1 Antitrypsin antibody [G11] 画像

  • Western blot using ab9400 at a concentration of 0.5 µg/ml.
    Lane 1 - purified alpha 1 antitrypsin
    lane 2 - human plasma, 2 µl
    lane S - standard proteins
    A - SDS-PAGE with Coomasie staining
    B - immunoblot with ab9400.

  • Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution + Albumin depleted human patient sera, tissue lysate at 50 µg/ml

    Secondary
    HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution

    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Mr John Andersen

    See Abreview

Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) 使用論文

This product has been referenced in:
  • Burgener A  et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . Read more (PubMed: 23449785) »
  • Lu J  et al. A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro. Mol Ther 21:954-63 (2013). WB ; Human . Read more (PubMed: 23459514) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human patient sera)
Loading amount 50 µg
Specification Human patient sera
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. John Andersen

Verified customer

投稿 Jul 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"