製品の概要

  • 製品名Anti-alpha 1 Antitrypsin antibody (FITC)
    alpha 1 Antitrypsin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to alpha 1 Antitrypsin (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with alpha 1 antitrypsin. This antibody may cross react with alpha 1 antitrypsin light chain from other species.
  • アプリケーション適用あり: Flow Cyt, ICC, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Unfortunately, this information is considered to be commercially sensitive

  • ポジティブ・コントロール
    • HepG2 cell line IF/ICC

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 21060150ab37394-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC 1/200 - 1/2000.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 組織特異性Ubiquitous. Expressed in leukocytes and plasma.
  • 関連疾患Alpha-1-antitrypsin deficiency
  • 配列類似性Belongs to the serpin family.
  • ドメインThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻訳後修飾N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 細胞内局在Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Dom1 antibody
    • PI antibody
    • PI1 antibody
    • PRO2275 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • Serpina1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Anti-alpha 1 Antitrypsin antibody (FITC) 画像

  • ICC/IF image of ab19170 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19170, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha 1 Antitrypsin antibody (FITC) (ab19170) 使用論文

This product has been referenced in:
  • Bergin DA  et al. The circulating proteinase inhibitor a-1 antitrypsin regulates neutrophil degranulation and autoimmunity. Sci Transl Med 6:217ra1 (2014). Read more (PubMed: 24382893) »
  • Carroll TP  et al. Evidence for unfolded protein response activation in monocytes from individuals with alpha-1 antitrypsin deficiency. J Immunol 184:4538-46 (2010). ICC/IF ; Human . Read more (PubMed: 20228200) »

See all 3 Publications for this product

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Ab19170, ab46533 and ab117851 have all been validated in Flow Cyt and therefore are guaranteed to work in this application.

Regarding ab19170, cells were were fixed with 4% paraformaldehyde but not perm...

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