Anti-alpha 1 Antichymotrypsin 抗体 (ab107362)

製品の概要

  • 製品名Anti-alpha 1 Antichymotrypsin antibody
    alpha 1 Antichymotrypsin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to alpha 1 Antichymotrypsin
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human alpha 1 Antichymotrypsin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Tissue lysates: Human Spinal cord; Human Colon; Hu Brain Frontal lobe (Alzheimers); Hu Plasma total protein. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • 組織特異性Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • 関連疾患Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • 配列類似性Belongs to the serpin family.
  • ドメインThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • SERPINA3 antibody
    • AACT antibody
    • AACT_HUMAN antibody
    • ACT antibody
    • Alpha-1-antichymotrypsin His-Pro-less antibody
    • Antichymotrypsin antibody
    • Cell growth inhibiting gene 24/25 protein antibody
    • Cell growth-inhibiting gene 24/25 protein antibody
    • GIG24 antibody
    • GIG25 antibody
    • Growth inhibiting protein 24 antibody
    • Growth inhibiting protein 25 antibody
    • MGC88254 antibody
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
    • Serine proteinase inhibitor clade A member 3 antibody
    • Serpin A3 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
    • SERPINA3 antibody
    see all

Anti-alpha 1 Antichymotrypsin antibody 画像

  • All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab107362) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Brain Frontal Lobe (Human)Tissue Lysate - Alzheimer
    Lane 4 : Human Plasma Total Protein Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 48 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 83 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of alpha 1 Antichymotrypsin staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107362, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-alpha 1 Antichymotrypsin antibody (ab107362) 使用論文

ab107362 has not yet been referenced specifically in any publications.

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