Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16 mice tumor cells)
Permeabilization
Yes - 0.25% Triton-X100 10 minutes
Specification
B16 mice tumor cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBST
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alexa Fluor 555) preadsorbed
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 19 2017