Application
Western blot
Sample
Human Cell lysate - whole cell (OV90 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount
1e+006 cells
Specification
OV90 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Concentration
0.5 µg/ml
Detection
Detection method
BioRad Clarity Western ECL
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 112 kDa
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投稿 Dec 06 2016