Anti-alpha 1 Fetoprotein 抗体 [1G7] (ab54745)
Key features and details
- Mouse monoclonal [1G7] to alpha 1 Fetoprotein
- Suitable for: WB, IHC-P, Sandwich ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-alpha 1 Fetoprotein antibody [1G7]
alpha 1 Fetoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1G7] to alpha 1 Fetoprotein -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Sandwich ELISA, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 500-610 of Human alpha 1 Fetoprotein
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特記事項
This product was changed from ascites to tissue culture supernatant on 13 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40 -
Concentration information loading...
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特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
1G7 -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54745の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection. |
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
IHC-P
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
組織特異性
Plasma. Synthesized by the fetal liver and yolk sac. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
発生段階
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻訳後修飾
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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別名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
画像
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alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
This image was generated using the ascites version of the product.
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alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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ab54745 at 10 ug/ml staining alpha Fetoprotein in human HeLa cells by Immunocytochemistry / Immunofluorescence.
This image was generated using the ascites version of the product.
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Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab54745 は 3 報の論文で使用されています。
- Er E et al. Metal Nanoparticles/MoS2 Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for a-Fetoprotein Detection. ACS Appl Mater Interfaces 13:8823-8831 (2021). PubMed: 33583183
- Lee NE et al. Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT). Commun Biol 3:636 (2020). PubMed: 33128033
- Maruyama S et al. Inhibition of apoptosis by miR-122-5p in a-fetoprotein-producing gastric cancer. Oncol Rep 41:2595-2600 (2019). PubMed: 30816512