Rabbit polyclonal to ALP
Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PQTVILPGPA PWGFRLSGGI DFNQPLVITR ITPGSKAAAA NLCPGDVILA) of human ALP (NP_055291).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
May play a role in the organization of actin filament arrays within muscle cells.
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
Information by UniProt
Actinin associated LIM protein antibody
Actinin-associated LIM protein antibody
Alpha actinin 2 associated LIM protein antibody
Western blot - ALP antibody (ab83259)
Anti-ALP antibody (ab83259) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
40 kDa (
why is the actual band size different from the predicted?
Additional bands at:
75 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"