Mouse polyclonal to ALP
Full length human protein (AAH27870, 1 a.a. ~ 317 a.a)
ALP transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
May play a role in the organization of actin filament arrays within muscle cells.
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
Information by UniProt
Actinin associated LIM protein antibody
Actinin-associated LIM protein antibody
Alpha actinin 2 associated LIM protein antibody
Western blot - ALP antibody (ab67228)
All lanes :
Anti-ALP antibody (ab67228) at 1/500 dilution
Lane 1 :
ALP transfected 293T cell lysate
Lane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size:
Observed band size:
35 kDa (
why is the actual band size different from the predicted?
This product has been referenced in:
et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016).
Read more (PubMed: 27432616) »
et al. Osteoinduction and proliferation of bone-marrow stromal cells in three-dimensional poly (e-caprolactone)/ hydroxyapatite/collagen scaffolds. J Transl Med 13:152 (2015).
Read more (PubMed: 25952675) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"