製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ALMS1
  • 特異性
    The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. ALMS1 protein has got a molecular weight of approximately 460kD. ab4306 results in a WB band of the right size, confirming that this antibody recognises the right protein.
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide: RVTNQLLGRKVPWD, corresponding to C terminal amino acids 4145-4167 of Human ALMS1.

  • 特記事項
    GenBank Accession Number – NP_055935. LocusLink ID - 7840 (human); 236266 (mouse).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Detects a band of approximately 460 kDa (predicted molecular weight: 460 kDa).Can be blocked with Human ALMS1 peptide (ab23023).
IHC-P Use a concentration of 1 - 3 µg/ml.

ターゲット情報

  • 機能
    Possible role in intracellular trafficking.
  • 組織特異性
    Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).
  • 関連疾患
    Defects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome.
  • 発生段階
    Widely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alms1 antibody
    • ALMS1_HUMAN antibody
    • ALSS antibody
    • Alstrom syndrome 1 antibody
    • Alstrom syndrome protein 1 antibody
    see all

Anti-ALMS1 antibody 画像



  • Predicted band size : 460 kDa


    20 µg of Hela extract were run on a Tris Acetate NuPage 3-8% gel and probed with a 1:200 dilution of the ab4306 antibody.

    The 460kD band represents ALMS1.
  • ab4306 (2µg/ml) staining of paraffin embedded Human Testis.

Anti-ALMS1 antibody (ab4306) 使用論文

ab4306 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
100 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Ankita

Verified customer

投稿 Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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