製品の概要

  • 製品名Anti-ALMS1 antibody
    ALMS1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to ALMS1
  • 特異性The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. ALMS1 protein has got a molecular weight of approximately 460kD. ab4306 results in a WB band of the right size, confirming that this antibody recognises the right protein.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide: RVTNQLLGRKVPWD, corresponding to C terminal amino acids 4145-4167 of Human ALMS1.

  • 特記事項GenBank Accession Number – NP_055935. LocusLink ID - 7840 (human); 236266 (mouse).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab4306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Detects a band of approximately 460 kDa (predicted molecular weight: 460 kDa).Can be blocked with Human ALMS1 peptide (ab23023).
IHC-P Use a concentration of 1 - 3 µg/ml.

ターゲット情報

  • 機能Possible role in intracellular trafficking.
  • 組織特異性Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).
  • 関連疾患Defects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome.
  • 発生段階Widely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alms1 antibody
    • ALMS1_HUMAN antibody
    • ALSS antibody
    • Alstrom syndrome 1 antibody
    • Alstrom syndrome protein 1 antibody
    see all

Anti-ALMS1 antibody 画像



  • Predicted band size : 460 kDa


    20 µg of Hela extract were run on a Tris Acetate NuPage 3-8% gel and probed with a 1:200 dilution of the ab4306 antibody.

    The 460kD band represents ALMS1.
  • ab4306 (2µg/ml) staining of paraffin embedded Human Testis.

Anti-ALMS1 antibody (ab4306) 使用論文

ab4306 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Rat Tissue lysate - whole (kidney)
Specification kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Ankita

Verified customer

投稿 Oct 29 2014

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. So far, it has been tested in ELISA ( peptide ELISA (antibody detection limit dilution >1 / 32000) and no signal obtained yet in We...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"