製品の概要

  • 製品名Anti-ALKBH5 antibody
    ALKBH5 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to ALKBH5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human OFOXD protein (NP_060228).

  • ポジティブ・コントロール
    • OFOXD transfected 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 関連性Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export and is required for spermatogenesis.
  • 細胞内局在Nucleus speckle
  • 参照データベース
  • 別名
    • ABH5 antibody
    • AlkB, alkylation repair homolog 5 (E. coli) antibody
    • AlkB, alkylation repair homolog 5 antibody
    • Alkylated DNA repair protein alkB homolog 5 antibody
    • Alpha ketoglutarate dependent dioxygenase alkB homolog 5 antibody
    • OFOXD antibody
    • OFOXD1 antibody
    • Oxoglutarate and iron-dependent oxygenase domain containing antibody
    • Probable alpha ketoglutarate dependent dioxygenase ABH5 antibody
    • RNA demethylase ALKBH5 antibody
    see all

Anti-ALKBH5 antibody 画像

  • All lanes : Anti-ALKBH5 antibody (ab69325) at 1/500 dilution

    Lane 1 : ALKBH5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa

Anti-ALKBH5 antibody (ab69325) 使用論文

This product has been referenced in:
  • Karkhanis V  et al. Protein Arginine Methyltransferase 7 Regulates Cellular Response to DNA Damage by Methylating Promoter Histones H2A and H4 of the Polymerase d Catalytic Subunit Gene, POLD1. J Biol Chem 287:29801-14 (2012). WB ; Human . Read more (PubMed: 22761421) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I am happy to hear that the positive control has helped with your other antibodies. Yes, K-562 cell lysate will be a suitable positive control for ALKBH5 as well. I hope this helps, please let me know if you have any other que...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"