Anti-ALKBH1 抗体 [hABH1-151] (ab49738)

製品の概要

  • 製品名Anti-ALKBH1 antibody [hABH1-151]
    ALKBH1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [hABH1-151] to ALKBH1
  • 特異性This antibody does not recognize hABH2 or hABH3.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human ALKBH1 aa 19-34 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GE DAFRKLFRFY RQSR

  • ポジティブ・コントロール
    • A total cell extract of 293T cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • ポリ/モノモノクローナル
  • クローン名hABH1-151
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 kDa.

ターゲット情報

  • 機能Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • 細胞内局在Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABH antibody
    • ABH1 antibody
    • alkB antibody
    • alkB homolog 1 histone H2A dioxygenase antibody
    • AlkB, alkylation repair homolog 1 (E. coli) antibody
    • ALKB1_HUMAN antibody
    • ALKBH antibody
    • ALKBH1 antibody
    • Alkylated DNA repair protein alkB homolog 1 antibody
    • Alkylation repair homolog 1 antibody
    • Alkylation repair, alkB homolog antibody
    • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
    • DNA lyase ABH1 antibody
    • DNA oxidative demethylase ALKBH1 antibody
    • hABH antibody
    see all

Anti-ALKBH1 antibody [hABH1-151] (ab49738) 使用論文

ab49738 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Rat Tissue lysate - whole (heart, brain, muscle)
Specification heart, brain, muscle
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Dec 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"