リコンビナント
RabMAb

Anti-Alkaline Phosphatase, Tissue Non-Specific 抗体 [EPR4477] (ab108337)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.

ターゲット情報

  • 機能
    This isozyme may play a role in skeletal mineralization.
  • 関連疾患
    Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • 配列類似性
    Belongs to the alkaline phosphatase family.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

画像

  • ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human liver tissue by Immunohistochemistry.

  • ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human kidney tissue by Immunohistochemistry.

  • ab108337, at a 1/100 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in HeLa cells by Immunofluorescence.

  • Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108337, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] (ab108337) at 1/10000 dilution

    Lane 1 : SAOS2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa

参考文献

This product has been referenced in:
  • Du M  et al. Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 70:62-70 (2017). WB ; Rat . Read more (PubMed: 27770935) »
  • Shen XQ  et al. Magnitude-dependent response of osteoblasts regulated by compressive stress. Sci Rep 7:44925 (2017). Read more (PubMed: 28317941) »

See all 13 Publications for this product

レビューと Q&A

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Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HeLa cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Oct 27 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
None
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Oct 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2-OS)
Loading amount
25 µg
Specification
U2-OS
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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