Anti-Alkaline Phosphatase, Tissue Non-Specific 抗体 [EPR4477] (ab108337)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.

ターゲット情報

  • 機能This isozyme may play a role in skeletal mineralization.
  • 関連疾患Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • 配列類似性Belongs to the alkaline phosphatase family.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] 画像

  • ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human liver tissue by Immunohistochemistry.

  • ab108337, at a 1/250 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human kidney tissue by Immunohistochemistry.

  • ab108337, at a 1/100 dilution, staining Alkaline Phosphatase, Tissue Non-Specific in HeLa cells by Immunofluorescence.

  • Overlay histogram showing Saos 2 cells stained with ab108337 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108337, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Saos 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] (ab108337) at 1/10000 dilution

    Lane 1 : SAOS2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 57 kDa

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [EPR4477] (ab108337) 使用論文

This product has been referenced in:
  • Du M  et al. Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 70:62-70 (2017). WB ; Rat . Read more (PubMed: 27770935) »
  • Huang X  et al. Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro. Mol Med Rep 13:613-22 (2016). WB ; Mouse . Read more (PubMed: 26648136) »

See all 4 Publications for this product

Product Wall

Thank you very much for your interest in ab108337.

To our knowledge, ab108337 has not been tested in flow cytometry. We do have four antibodies against bone alkaline phosphatase. Only the ab108337 has been tested against the rat protein and ...

Read More
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Heart)
Antigen retrieval step None
Specification Heart
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 26 2016

We unfortunately do not have any data from ISPC for this ALP antibody. We do have another ALP tissue non-specific antibody that has been used to stain undifferentiated human embryonic stem cells (H9), but this antibody, ab17973, is non-reactive with mo...

Read More

Thank you for contacting us.

This is correct. You will receive a vial of 100 ul with xxxxx ug of ab108337 antibody in it. This may appear like a small amount but this is because it is a rabbit monoclonal antibody which has a high affinity fo...

Read More

Thank you for contacting us.

The concentration for Anti-Bone Alkaline Phosphatase antibody [EPR4477] (ab108337), lot GR79855-5 in stock is xxxxxx mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact ...

Read More

Thank you for contacting Abcam.
Although the exact immnuogen sequences are considered to be proprietary, I can let you the following information narrowing down the regions that these antibodies recognize:
- ab108337 - Between ***
- ab658...

Read More

Thank you for your enquiry and interest in our products.

I can confirm that both antibodies (ab22552 and ab108337) have been tested in IHC-P and recognize the murine proteins (Osterix and Bone Alkaline Phosphatase, respectively). This means t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2-OS)
Loading amount 25 µg
Specification U2-OS
Gel Running Conditions Reduced Denaturing (15%)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2012

Vielen Dank für Ihre Anfrage.


Leider muss ich Sie bezüglich der Konzentration enttäuschen: Es handelt sich hier um einen unaufgreinigten Zellkulturüberstand, bei dem es unmöglich ist, die eigentliche Antikö...

Read More

Thats no problem.
As I mentioned in my previous email, if you do have any further problems or questions, please do not hesitate to contact us again.
Until then, I wish you all the best with your research.

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"