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2102Ep Human Embryonal Carcinoma Cells
Our Abpromise guarantee covers the use of ab17973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/50. PubMed: 23225236|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.
Dilution used: 1/50
Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.