Anti-Alkaline Phosphatase, Tissue Non-Specific 抗体 [3H414 (TRA-2-49)] (ab17973)


  • 製品名Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)]
    Alkaline Phosphatase, Tissue Non-Specific 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3H414 (TRA-2-49)] to Alkaline Phosphatase, Tissue Non-Specific
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 種交差性
    交差種: Rabbit, Cat, Human, Pig, Chimpanzee, Gorilla, Hoolock Gibbon, Orangutan, Owl monkey
    非交差種: Mouse, Rat, Sheep, Goat, Guinea pig, Hamster, Cow, Dog, Baboon, Rhesus monkey
  • 免疫原

    2102Ep Human Embryonal Carcinoma Cells

  • 特記事項Clone 3H414 was previously known as TRA-2-49/6E.



Our Abpromise guarantee covers the use of ab17973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50. PubMed: 23225236
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.


  • 機能This isozyme may play a role in skeletal mineralization.
  • 関連疾患Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • 配列類似性Belongs to the alkaline phosphatase family.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] 画像

  • Undifferentiated human embryonic stem cells (H9 line) were stained with the tissue non-specific alkaline phosphatase antibody, ab17973. The cells were positive for alkaline phosphatase with staining localised on the surface membrane.

    Dilution used: 1/50

    Note that the feeder cells in the bottom left hand corner of the plate are not stained by this antibody.

  • ab17973 staining Alkaline Phosphatase, Tissue Non-Specific in human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [3H414 (TRA-2-49)] (ab17973) 使用論文

This product has been referenced in:
  • Alves RD  et al. Calcifying vascular smooth muscle cells and osteoblasts: independent cell types exhibiting extracellular matrix and biomineralization-related mimicries. BMC Genomics 15:965 (2014). Flow Cyt ; Human . Read more (PubMed: 25380738) »
  • Aliagas E  et al. Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium. Purinergic Signal 9:227-37 (2013). IHC-Fr ; Human . Read more (PubMed: 23225236) »

See all 5 Publications for this product

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The lab has tested ab17973 with H9 cells, NTERA-2 cl.D1 cells, iPSCs and A431 cells, while ab17272 has been tested with U2-OS cells. So, any of these cells can be used as positive controls.

Ab17973 was tested in the lab using the following pro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal gland)
Specification Adrenal gland
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jun 01 2011

Thank you for your enquiry and your patience. I'm afraid this antibody is not specific for leukocyte alkaline phosphatase. It is only known to react with the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Also, this antibody does not cro...

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