A KLH conjugated synthetic peptide selected from the center region of human Alkaline Phosphatase.
Our Abpromise guarantee covers the use of ab75699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 58 kDa). | |
ELISA | 1/1000. | |
IHC-P | 1/10 - 1/50. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human hepatocarcinoma tissue sections labeling Alkaline Phosphatase with ab75699 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
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