Anti-Alkaline Phosphatase 抗体 (ab75699)
製品の概要
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製品名Anti-Alkaline Phosphatase antibody
Alkaline Phosphatase 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Alkaline Phosphatase
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由来種Rabbit
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アプリケーション適用あり: WB, ELISA, IHC-Pmore details
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種交差性交差種: Human
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免疫原
A KLH conjugated synthetic peptide selected from the center region of human Alkaline Phosphatase.
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ポジティブ･コントロール
- This antibody gave a positive signal in human liver tissue lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
精製度Ammonium Sulphate Precipitation
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特記事項（精製）This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab75699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 58 kDa). | |
| ELISA | 1/1000. | |
| IHC-P | 1/10 - 1/50. |
ターゲット情報
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配列類似性Belongs to the alkaline phosphatase family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 248 Human
- Entrez Gene: 249 Human
- Entrez Gene: 250 Human
- Entrez Gene: 251 Human
- Omim: 171740 Human
- Omim: 171760 Human
- Omim: 171800 Human
- Omim: 171810 Human
see all -
別名
- Alkaline phosphatase, germ cell antibody
- Alkaline phosphatase, testicular and thymus antibody
- Akp2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase antibody (ab75699)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human hepatocarcinoma tissue sections labeling Alkaline Phosphatase with ab75699 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
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Western blot - Anti-Alkaline Phosphatase antibody (ab75699)Anti-Alkaline Phosphatase antibody (ab75699) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
Exposure time: 2 minutes
Alkaline Phosphatase protein contains a 22-amino acid signal peptide and a 29-amino acid propeptide, both of which are removed in the mature form (SwissProt). This might explain the migration of the band at a lower molecular weight than predicted.
参考文献
This product has been referenced in:
- Ng JJ et al. Recapitulation of physiological spatiotemporal signals promotes in vitro formation of phenotypically stable human articular cartilage. Proc Natl Acad Sci U S A 114:2556-2561 (2017). IHC-P ; Human . Read more (PubMed: 28228529) »
- Andersen TL et al. Understanding coupling between bone resorption and formation: are reversal cells the missing link? Am J Pathol 183:235-46 (2013). Read more (PubMed: 23747107) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"