Customer reviews and FAQs

製品の概要

  • 製品名Anti-Alkaline Phosphatase antibody
    Alkaline Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Alkaline Phosphatase
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Escherichia coli
    非交差種: Mammal
  • 免疫原

    Full length protein (E. coli).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/8000 - 1/40000.
IP Use at an assay dependent dilution.
ELISA 1/8000 - 1/40000.

ターゲット情報

  • 配列類似性Belongs to the alkaline phosphatase family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 別名
    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase antibody 画像

  • ELISA - Alkaline Phosphatase antibody (ab354)
    ELISA - Alkaline Phosphatase antibody (ab354)This image is courtesy of an anonymous Abreview
    Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.

    See Abreview

  • Western blot - Alkaline Phosphatase antibody (ab354)
    Western blot - Alkaline Phosphatase antibody (ab354)
    All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilution

    Lane 1 : Escherichia coli Recombinant protein (Mach1)
    Lane 2 : Escherichia coli Recombinant protein (Mach1)

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    An HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilution
    Developed using the ECL technique

    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    Image courtesy of an anonymous Abreview.

Anti-Alkaline Phosphatase antibody (ab354) 使用論文

This product has been referenced in:
  • Bross P  et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB ; Escherichia coli . Read more (PubMed: 18400758) »
  • Imperi F  et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008). Read more (PubMed: 18757814) »

See all 3 Publications for this product

ab354 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab354 の abreview を投稿する ab354 に関する問い合わせをする

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ELISA abreview for Anti-Alkaline Phosphatase antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Escherichia coli Recombinant protein (Mach1)
Specification Mach1
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type Indirect
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Verified customer

投稿 Feb 26 2010

Western blot abreview for Anti-Alkaline Phosphatase antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (Mach1)
Loading amount 0.5 µg
Specification Mach1
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
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Verified customer

投稿 Feb 19 2010

Please help with the following questions: What is the concentration of the antibody to bacterial alkaline phosphatase? Is this an affinity purified polyclonal? Are there ANY other proteins in the preparation?

Thank you for your enquiry. This product is sold as tissue culture supernatant and not affinity purified. As a tissue culture supernatant this product will contain additional proteins and the IgG concentration has not been determined. Typically IgG con...

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I saw an enquiry dated 7-Feb-2001 asking whether this antibody can detect secreted alk phos (SEAP). At the time, the answer was that you have no reports -- have you heard anything further about this application since?

This antibody should detect SEAP from E.coli. It has not been tested for cross-reactivity in other bacteria and does not cross-react with AP from mammalian species.

I'm having trouble using this product for ELISA at 2µg/ml. Please let me know the recommended conditions.

A working dilution from our test using lot 4679(assayed againest 1.0 ug of Alkaline Phosphatase in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anit- Rabbit IgG, was 1:8,000 to 1:40,000 (volume:volume).

We want to use alkaline phosphatase antibody to determine ALP releasing in periodontal cells (Osteoblasts, PDL fibroblasts and cementoblasts). Does Alp1-ab354 test ALP expression in bone-like cells by immunohistochemistry?

No this antibody is not suitable as it does not cross react with the mammalian form of Alkaline Phosphatase. The immunogen is Alkaline Phosphatase from E.coli. We suggest you review our other alkaline phosphatase antibodies, many of which have been tes...

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I would like to know whether your alkaline phosphatase antibody is a marker of Plasma membrane or not.

No, this antibody is not a marker of plasma membrane.

Do you know if your Alp1 Ab can detect secreted alkaline phosphatase (SEAP)?

We have no reports of it being used in this application, but do not see a reason why it should not recognise SEAP.

We want to use alkaline phosphatse as a marker in E. coli cell fraction experiments. To monitor alk. phosphatase we want to use your antibody. Do you know if alk. phosphatase is expressed under all condition in E. coli (at different levels). Many than...

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We believe it is although it might be more highly expressed in stationary phase and be strain dependent. Abcam

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