製品の概要

  • 製品名Anti-ALIX antibody [3C4]
    ALIX 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3C4] to ALIX
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human ALIX aa 1-110.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
    • 配列類似性Contains 1 BRO1 domain.
    • 細胞内局在Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AIP1 antibody
      • ALG 2 interacting protein 1 antibody
      • ALG-2-interacting protein 1 antibody
      • ALG2 interacting protein X antibody
      • Alix antibody
      • Apoptosis linked gene 2 interacting protein X antibody
      • Dopamine receptor interacting protein 4 antibody
      • DRIP4 antibody
      • Hp95 antibody
      • KIAA1375 antibody
      • MGC17003 antibody
      • PDC6I_HUMAN antibody
      • PDCD6 interacting protein antibody
      • PDCD6-interacting protein antibody
      • PDCD6IP antibody
      • Programmed cell death 6 interacting protein antibody
      • Programmed cell death 6-interacting protein antibody
      see all

    Anti-ALIX antibody [3C4] 画像

    • Western blot against tagged recombinant protein immunogen using ab56932 PDC6I antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa
    • All lanes : Anti-ALIX antibody [3C4] (ab56932) at 3 µg/ml

      Lane 1 : Cell lysates prepared from human Vero cells
      Lane 2 : Cell lysates prepared from human Vero cells

      Lysates/proteins at 20000 cells per lane.

      Secondary
      HRP-conjugated sheep polyclonal to mouse IgG at 1/10000 dilution

      Performed under reducing conditions.

      Observed band size : 95 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      This image is a courtesy of Anonymous Abreview

      See Abreview

    Anti-ALIX antibody [3C4] (ab56932) 使用論文

    ab56932 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vero)
    Loading amount 20000 cells
    Specification vero
    Treatment cells expressing recombitant PDC6I
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Jun 23 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"