製品の概要

  • 製品名
    Anti-ALIX antibody [3A9]
    ALIX 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3A9] to ALIX
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis, Xenopus tropicalis
  • 免疫原

    Fusion protein corresponding to Human ALIX.

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
  • 配列類似性
    Contains 1 BRO1 domain.
  • 細胞内局在
    Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIP1 antibody
    • ALG 2 interacting protein 1 antibody
    • ALG-2-interacting protein 1 antibody
    • ALG2 interacting protein X antibody
    • Alix antibody
    • Apoptosis linked gene 2 interacting protein X antibody
    • Dopamine receptor interacting protein 4 antibody
    • DRIP4 antibody
    • Hp95 antibody
    • KIAA1375 antibody
    • MGC17003 antibody
    • PDC6I_HUMAN antibody
    • PDCD6 interacting protein antibody
    • PDCD6-interacting protein antibody
    • PDCD6IP antibody
    • Programmed cell death 6 interacting protein antibody
    • Programmed cell death 6-interacting protein antibody
    see all

画像

  • Anti-ALIX antibody [3A9] (ab117600) + HeLa whole cell lysate

    Secondary
    Rabbit Anti Mouse IgG

    Predicted band size : 96 kDa

参考文献

This product has been referenced in:
  • Nakata R  et al. Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells. J Extracell Vesicles 6:1332941 (2017). Read more (PubMed: 28717423) »
  • Dean I  et al. The secretion and biological function of tumor suppressor maspin as an exosome cargo protein. Oncotarget 8:8043-8056 (2017). Read more (PubMed: 28009978) »

See all 5 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Epethelial cells)
Specification
Epethelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

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Verified customer

投稿 Aug 20 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (Epethelial cells)
Specification
Epethelial cells
Total protein in input
250 µg
Username

Abcam user community

Verified customer

投稿 Aug 20 2014

Thank you for contacting us.

Here is the WB protocol you had requested.

Western Blotting

Reagents
1. Blocking Buffer
5% non-fat dried milk dissolved in PBS.
Note: For the detection of phosphoproteins, NEVE...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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