製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ALG6
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (GDYEAQRHWQ EITFNLPVKQ WYFNSSDNNL QYWGLDYPPL TAYHSLLCAY) of human ALG6 (NP_037471)

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab80873 is purified by a peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 関連性
    ALG6 (Asparagine-linked glycosylation 6 homolog) is a Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase which catalyzes the addition of the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. It transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man(9)GlcNAc(2)-PP-Dol.
  • 細胞内局在
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • Asparagine linked glycosylation 6 antibody
    • Asparagine linked glycosylation 6 homolog (S. cerevisiae alpha 1 3 glucosyltransferase) antibody
    • Asparagine linked glycosylation 6 homolog antibody
    • Dolichyl P Glc:Man9GlcNAc2 PP dolichyl glucosyltransferase antibody
    • Dolichyl P Glc:Man9GlcNAc2 PP dolichylglucosyltransferase antibody
    • Dolichyl pyrophosphate Man9GlcNAc2 alpha 1 3 glucosyltransferase antibody
    • E230028F23Rik antibody
    • Man(9)GlcNAc(2) PP Dol alpha 1 3 glucosyltransferase antibody
    • MGC124652 antibody
    • My 046 antibody
    • My046 antibody
    • OTTMUSP00000008309 antibody
    • RP23-35D18.4 antibody
    see all

画像

  • Anti-ALG6 antibody (ab80873) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

プロトコール

参考文献

ab80873 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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