製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ALDH9A1
  • アプリケーション
    適用あり: WB, Indirect ELISA, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human)from the C-terminal region of human ALDH9A1 conjugated to KLH.

  • ポジティブ・コントロール
    • Mouse kidney tissue lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 54 kDa.
Indirect ELISA 1/1000.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction.
  • 組織特異性
    High expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain. Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole.
  • パスウェイ
    Amine and polyamine biosynthesis; carnitine biosynthesis.
  • 配列類似性
    Belongs to the aldehyde dehydrogenase family.
  • 発生段階
    Strongly expressed in human embryonic brain (gestational age 12 weeks).
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4 trimethylaminobutyraldehyde dehydrogenase antibody
    • 4-trimethylaminobutyraldehyde dehydrogenase antibody
    • AL9A1_HUMAN antibody
    • aldehyde dehydrogenase (NAD+) antibody
    • Aldehyde dehydrogenase 9A1 antibody
    • Aldehyde dehydrogenase E3 isozyme antibody
    • Aldehyde dehydrogenase family 9 member A1 antibody
    • ALDH4 antibody
    • ALDH7 antibody
    • ALDH9 antibody
    • aldh9A1 antibody
    • E3 antibody
    • Gamma aminobutyraldehyde dehydrogenase antibody
    • Gamma-aminobutyraldehyde dehydrogenase antibody
    • R aminobutyraldehyde dehydrogenase antibody
    • R-aminobutyraldehyde dehydrogenase antibody
    • TMABADH antibody
    see all

画像

  • Anti-ALDH9A1 antibody (ab79726) at 1/50 dilution + mouse kidney tissue lysate at 35 µg

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Immunoprecipitation
Sample
Human Tissue lysate - whole (brain)
Total protein in input
500 µg
Specification
brain
Immuno-precipitation step
Other - dynabead
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Verified customer

投稿 May 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 May 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (BRAIN)
Loading amount
50 µg
Specification
BRAIN
Gel Running Conditions
Non-reduced Denaturing (12%PA GEL)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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投稿 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (blood)
Specification
blood
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
Type
Direct
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投稿 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell line HpL3-4)
Loading amount
30 µg
Specification
neuronal cell line HpL3-4
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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投稿 Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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