Anti-ALDH7A1 抗体 [EP1935Y] (ab53278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1935Y] to ALDH7A1
- Suitable for: IP, Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ALDH7A1 antibody [EP1935Y]
ALDH7A1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1935Y] to ALDH7A1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, Flow Cyt, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, HT-29, and Human, Mouse, and Rat liver lysate. IHC-P: Human, Mouse, and Rat liver tissue. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: Human liver.
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特記事項
This product has switched from a hybridoma to recombinant production method on 7th September 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1935Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
1/20.
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Flow Cyt |
1/20.
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ICC/IF |
1/50.
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WB |
1/10000 - 1/50000.
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IHC-P |
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
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IP
1/20. |
Flow Cyt
1/20. |
ICC/IF
1/50. |
WB
1/10000 - 1/50000. |
IHC-P
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Entrez Gene: 291450 Rat
- Omim: 107323 Human
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
- SwissProt: Q64057 Rat
- Unigene: 483239 Human
see all -
別名
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
画像
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Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700) at 1/10000 dilution + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-ALDH7A1 antibody [EP1935Y] (ab53278) at 1/2000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Human liver lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with ab53278 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab53278 overnight at 4° C. Nucleus were visualized using DAPI.
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HepG2 cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab53278 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.
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ALDH7A1 was immunoprecipitated from 0.35 mg of human liver tissue lysate with the antibody at 1/20 dilution. Western blot was performed from the immunoprecipitated using antibody at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used as secondary antibody at 1/5000 dilution.
Lane 1: Human liver tissue lysate 10 μg.
Lane 2: Human liver tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab53278 in human liver tissue lysate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab53278 は 6 報の論文で使用されています。
- Lee JS et al. Overall survival of pancreatic ductal adenocarcinoma is doubled by Aldh7a1 deletion in the KPC mouse. Theranostics 11:3472-3488 (2021). PubMed: 33537098
- Al-Shekaili HH et al. A novel mouse model for pyridoxine-dependent epilepsy due to antiquitin deficiency. Hum Mol Genet 29:3266-3284 (2020). PubMed: 32969477
- Milone MR et al. Novel pathways involved in cisplatin resistance identified by a proteomics approach in non-small-cell lung cancer cells. J Cell Physiol 234:9077-9092 (2019). PubMed: 30362533
- Stoll G et al. Metabolic enzymes expressed by cancer cells impact the immune infiltrate. Oncoimmunology 8:e1571389 (2019). PubMed: 31069148
- Belvedere R et al. Annexin A1 contributes to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of Formyl Peptide Receptor pathway. Sci Rep 6:29660 (2016). PubMed: 27412958
- Milone MR et al. Proteomic analysis of zoledronic-acid resistant prostate cancer cells unveils novel pathways characterizing an invasive phenotype. Oncotarget 6:5324-41 (2015). WB . PubMed: 25481874