製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ALDH6A1
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    A KLH conjugated synthetic peptide from the N-terminal region of human ALDH6A1.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: T47D.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 58 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.
ICC/IF Use at an assay dependent concentration. PubMed: 20966424

ターゲット情報

  • 機能
    Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
  • 関連疾患
    Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
  • 配列類似性
    Belongs to the aldehyde dehydrogenase family.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110038I05Rik antibody
    • AI314632 antibody
    • Aldehyde dehydrogenase 6 family member A1 antibody
    • Aldehyde dehydrogenase family 6 member A1 antibody
    • Aldehyde dehydrogenase family 6, subfamily A1 antibody
    • ALDH6A1 antibody
    • Malonate semialdehyde dehydrogenase antibody
    • Malonate-semialdehyde dehydrogenase [acylating] antibody
    • Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial antibody
    • Methylmalonate-semialdehyde dehydrogenase [acylating] antibody
    • MGC37325 antibody
    • MGC40271 antibody
    • Mitochondrial acylating methylmalonate semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • MMSA_HUMAN antibody
    • MMSADHA antibody
    • MMSDH antibody
    see all

画像

  • ab63181 staining of human breast carcinoma tissue by formalin-fixed and parrafin-embedded IHC.    
  • Anti-ALDH6A1 antibody (ab63181) at 1/50 dilution + T47D cell lysate at 35 µg

    Predicted band size : 58 kDa
  • ICC/IF image of ab63181 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63181 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Suryawanshi AR  et al. Epididymosome Mediated Acquisition of MMSDH an Androgen Dependent and Developmentally Regulated Epididymal Sperm Protein. J Androl : (2011). WB, IHC-P ; Rat . Read more (PubMed: 22207704) »
  • Suryawanshi AR  et al. Differential Proteomics Leads to Identification of Domain Specific Epididymal Sperm Proteins. J Androl : (2010). WB, ICC/IF ; Human, Rat . Read more (PubMed: 20966424) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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