Mouse monoclonal [9A10AF10] to ALDH2
Mouse, Rat, Cow, Human
Tissue, cells or virus corresponding to ALDH2. Rat liver mitochondria.
HepG2 cells; Human, Rat and Mouse liver and HepG2 cell lysates.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.02% Sodium azide
Concentration information loading...
>95% by SDS-PAGE
ab110311 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. For 2 hours.
Use at an assay dependent dilution.
Use a concentration of 5 µg/ml.
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Belongs to the aldehyde dehydrogenase family.
Information by UniProt
Acetaldehyde dehydrogenase 2 antibody
Aldehyde dehydrogenase 2 family (mitochondrial) antibody
Aldehyde dehydrogenase 2 family antibody
Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10 ] (ab110311)
Immunocytochemistry analysis using ab110311 at 5µg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: Target protein locates mainly in the mitochondrial matrix.
Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10 ] (ab110311)
Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"