製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to ALDH1L1
  • アプリケーション
    適用あり: WB, IHC-P, IP, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Recombinant fragment: EESFGPVMII SRFADGDLDA VLSRANATEF GLASGVFTRD INKALYVSDK LQAGTVFVNT YNKTDVAAPF GGFKQSGFGK DLGEAALNEY LRVKTVTFEY , corresponding to amino acids 803-903 of Human ALDH1L1

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibodies ab87117 or ab177463 as replacements.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 99 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 組織特異性
    Highly expressed in liver, pancreas and kidney.
  • 配列類似性
    In the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

画像

  • ALDH1L1 antibody (ab56777) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.


  • Predicted band size : 99 kDa
    ALDH1L1 antibody (ab56777) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • ICC/IF image of ab56777 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56777, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab56777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56777, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ALDH1L1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to ALDH1L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56777.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 99kDa: ALDH1L1; non specific - 60kDa: We are unsure as to the identity of this extra band.

参考文献

This product has been referenced in:
  • Chen J  et al. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3:17044 (2017). Read more (PubMed: 29238610) »
  • Yuan JX  et al. Quantitative Analysis of the Cellular Microenvironment of Glioblastoma to Develop Predictive Statistical Models of Overall Survival. J Neuropathol Exp Neurol N/A:N/A (2016). Read more (PubMed: 27815396) »

See all 9 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Syrian Hamster Cell (Mixed glial culture)
Specification
Mixed glial culture
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Feb 15 2013

Considering that you would like to perform IHC on rabbit specimen, I agree that it is advisable to avoid antibodies generated in rabbit. Therefore, although the MMP12 antibody ab56305 shows a rather low sequence similarity across the full-length protei...

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Thank you very much for your interest in our antibodies. The immunogen and epitope sequences, respectively, show the following similarities against rabbit: 1) ab70191: 93% 2) ab56777: 96% 3) ab56305: 72% 4) ab6328: 100% Due to this high hom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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