Anti-ALDH1A1 抗体 [5A11] (ab105920)
Key features and details
- Mouse monoclonal [5A11] to ALDH1A1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ALDH1A1 antibody [5A11]
ALDH1A1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5A11] to ALDH1A1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Purified recombinant fragment of Human ALDH1A1 expressed in E. coli.
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ポジティブ・コントロール
- WB: Raji, Jurkat, THP1 and K562 cell lysates IHC-P: Human cerebellum and lung cancer tissues
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR120645-17 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
5A11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105920の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200 - 1/1000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/200 - 1/1000. Predicted molecular weight: 55 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
パスウェイ
Cofactor metabolism; retinol metabolism. -
配列類似性
Belongs to the aldehyde dehydrogenase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 216 Human
- Omim: 100640 Human
- SwissProt: P00352 Human
- Unigene: 76392 Human
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別名
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
画像
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ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human cerebellum tissue by Immunohistochemistry.
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Overlay histogram showing HepG2 cells stained with ab105920 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105920, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-ALDH1A1 antibody [5A11] (ab105920) at 1/200 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : K562 cell lysate
Predicted band size: 55 kDa -
ab105920, at a 1/200 dilution, staining ALDH1A1 in paraffin embedded Human lung cancer tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab105920 は 5 報の論文で使用されています。
- Gunduz M et al. Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer. Oncol Lett 19:965-971 (2020). PubMed: 31897209
- Sulaiman A et al. Dual inhibition of Wnt and Yes-associated protein signaling retards the growth of triple-negative breast cancer in both mesenchymal and epithelial states. Mol Oncol N/A:N/A (2018). WB . PubMed: 29316250
- Jia D et al. An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells. Cell Death Dis 8:e2932 (2017). PubMed: 28703802
- Jia D et al. Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo. Oncotarget 7:771-85 (2016). WB . PubMed: 26506421
- Martinez-Cruzado L et al. Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations. Sci Rep 6:27878 (2016). WB, IF . PubMed: 27292183