Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)

製品の概要

  • 製品名Aldehyde Quantification Assay Kit (Colorimetric)
    Aldehyde キット 製品一覧
  • テスト
    1 x 200 test
  • サンプルの種類
    Saliva, Plasma, Cell culture extracts, Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 種交差性
    交差種: Human
  • 製品の概要

    ab112113 Aldehyde Quantification Assay Kit (Colorimetric) uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. ab112113 provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm. This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ab112113 should be stored desiccated.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112113 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Aldehyde Quantification Assay Kit (Colorimetric) 画像

    • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

    • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

    • Aldehyde measured in biological fluids showing quantity (uM) per mL of tested sample. Values calculated based on the 550 nm Standard Curve. Samples diluted 1-10 fold.

    • Aldehyde measured in cell culture supernatants showing quantity (uM) per mL of tested sample. Values calculated based on the 405 nm Standard Curve. Samples diluted 2 fold.

    • Aldehyde measured in cell lysates showing quantity (uM) per 1 mln cells. Values calculated based on the 550 nm Standard Curve.

      Samples with the concentration of 1.26e8 cells/mL (HeLa) and 1e8 cells/mL (HepG2) were used. Samples were diluted 3 fold.

    • Aldehyde dose response was measured in a 96-well black plate with ab112113. As low as 10 µM (1 nanomol/well) of aldehyde can be detected with 30 minutes incubation (n=3). Standard curve was read at 405 nm.

    プロトコール

    Aldehyde Quantification Assay Kit (Colorimetric) (ab112113) 使用論文

    ab112113 has not yet been referenced specifically in any publications.

    Product Wall

    ab112113 detects all the aldehydes in solution. We don't have experiences in tissue samples, but it should be ok theoretically. Simply use your traditional homogenization method for sample preparation.

    For product ab112113, you measure the endpoint and not kinetic change over time. After 30-60 minutes of incubation this is when you can measure the OD at 405 or 550 nm depending on on the concentration of aldehyde in your samples. At lower concentratio...

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    The assay ab112113 is capable of detecting aldehydes in polysacharrides but we have not tested that sample type. The standard in the assay is glyceraldehyde which is relatively small, because the assay was developed to detect small aldehydes. So, detec...

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    Thank you for contacting us.



    1. The wavelength is mostly depending on the type of the aldehyde, and the incubation time. For example, Formaldehyde have only one peak 550nm, others have two peaks.

    2. Our standard is
    ...

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    The lab has not observed this before, and we have not received similar feedback from other customers. I would choose the values you obtained for the shorter assay incubation, rather than the overnight one. What you are seeing over time may in fact be t...

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    ab112113 can be used on plasma samples. The plasma samples don't need any special preparation so no spin columns are needed to deproteinise these samples.
    .

    The lab does not have experiences using this on fermentation broth. It should be OK theoretically, but you will need to subtract the background color of the broth from your reads.

    Hello and thank you for contacting us. The kit should work using the method you described, stopping the reaction, but we recommend using a basic solution (pH8) when doing so. Please let me know if you have any further questions.

    Thank you for your inquiry.
    I can confirm that the standard of ab112113 is Glyceradehyde (MW=90).
    I hope this information is helpful and that this kit is suitable for your experiments.

    Thank you for contacting us.
    Here are the answers to your questions:

    1) How much milligram of yellow indicator are there per vial? Answer: ˜ 24mg

    2) What is the exact chemistry of the kit ? Answer: The sensor contains an amine ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"